Serial cloner online9/6/2023 Students isolate and purify DNA using a plasmid purification protocol, and measure quantity and quality using a spectrophotometer. They are introduced to molecular cloning and the value of master mixes, beginning with the digestion of their DNA plasmid using restriction enzymes. Students, making their own buffers and gels, perform gel electrophoresis and subsequently cut-out the appropriate bands. Students purify bands and clean-up DNA with magnetic beads, preparing samples for the next step. Students perform DNA ligation with proper controls and transform ligations into DH5-α super competent cells. To verify successful transformation, students make Luria Broth ampicillin plates and spread transformed bacteria onto plates ligation efficiency is measured based on bacterial colony count. The students then use colony PCR and agarose gel analysis to verify successful ligations. At the end of the semester, students compile results from each step during the 11-week lab series and integrate learning from the lecture to synthesize a comprehensive lab report. Student evaluation is based on connecting lecture concepts to laboratory experiments, including clearly stating lab objectives, writing protocols in own words, accurately recording results in notebook (in pen), labeling of figures, analysis of data, and comprehension of results. Properly recording and understanding results is more highly valued over expected results. Successful experiments carried-out in the course are used to contribute to authentic research projects. The goal of this lab course is to integrate authentic research into the undergraduate learning experience, preparing students for graduate school and employment in a modern biotechnological lab establishing the next generation of scientist. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.Serial Cloner Alternatives and Similar Software | AlternativeTo (function()() Skip to main contentSkip to site searchPlatformsCategoriesOnlineWindowsAndroidMaciPhoneLinuxiPadAndroid TabletProductivitySocialDevelopmentBackupRemote Work & StudyLoginSign up HomeSerial ClonerAlternativesSerial Cloner AlternativesSerial Cloner is described as *'Molecular Biology software. It provides tools with an intuitive interface that assists you in DNA cloning, sequence analysd visualization'* and is an app. There are more than 25 alternatives to Serial Cloner for a variety of platforms, including Windows, Mac, Linux, Online / Web-based and Self-Hosted solutions. In conclusion, Serial Cloner is a very user-friendly and powerful Molecular biology tool.It provides tools with an intuitive interface that assists you in DNA.Learn more about Serial ClonerSerial Cloner info, screenshots & reviewsAlternatives to Serial Cloner25 FiltersMac + FreeShow more filters 3DNADynamoIs this a good alternative? Yes NoCopy a direct link to this comment to your clipboard Paid ProprietaryMacWindowsLinuxSerial Cloner is a Molecular Biology software.Other great apps like Serial Cloner are SnapGene Viewer, Benchling, Geneious and BioEdit. Suggest changesThis page was last updated Serial Cloner 2 Free It's not free, so if you're looking for a free alternative, you could try ApE - A plasmid Editor or UGENE. Fragments obtained by PCR, shRNA synthesis, can be easily assembled by selecting between the restriction sites.We can also ligate fragments using the ligate button.Also, to create new PCR based fragments or synthetic adaptors. We can use either the graphics window or the text-based site usage windows, which makes it very easy to quickly extract a sub-sequence in a selection or in any restriction sites.There is a web browser tool within the software to allow direct import of NCBI and EMBL.Sequence features are visible on the sequence map.They can be automatically found by using the scan feature. Features can be manually entered or imported.The fully graphical map makes it easy to extract and select a fragment or even show single, double, or multiple cutters in a single window. All the tools required to analyze the sequence and modify if any changes needed are available in a single window making it very easy to use.The software comes with a high-quality graphics display and has a very user-friendly interface to the front end, which makes the interface very intuitive. They are processed both in sequence and as a graphic map. Since version 2.0 of the software, it also handles Annotations and features. It can read and write files in several formats, making it portable across multiple users who use different Molecular Biology tools. It is available free for download and can be used across multiple Operating Systems, including Windows, Mac, and Linux. Serial Cloner is a Molecular Biology Tool which is a front end to help with DNA Cloning, Sequence Analysis, and Visualization.
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